Plasmid_Backbone

Part:BBa_K2170301:Design

Designed by: Max Mustermann   Group: iGEM16_LMU-TUM_Munich   (2016-10-12)


pcDNA5 for genomic integration


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 361
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4102
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4102
    Illegal BglII site found at 3880
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4102
    Illegal suffix found at 2
    Illegal XbaI site found at 361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4102
    Plasmid lacks a suffix.
    Illegal XbaI site found at 361
    Illegal XbaI site found at 4117
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 221
    Illegal NgoMIV site found at 1484
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3012
    Illegal SapI site found at 1929


Design Notes

none


Source

Chair for biological chemistry TUM

References